Observe closely the changes which occur in cobalt chloride papers placed on both the surfaces. Now the whole experimental setup is kept under bright sunlight. One strip of this dry blue cobalt chloride paper is kept on either surface of a healthy dorsiventral leaf and are covered by glass slides with the help of clips. Due to this characteristic feature it acts as a chemical indicator in this experiment. Cobalt chloride paper will be blue in color when it is dry and become pink when it absorbs moisture. Now two strips of filter paper are soaked in 2% solution of cobalt chloride and are perfectly dried under sunlight. Procedure for the experimentA well watered dicot plant has to be selected for this experiment. RequirementsA well watered potted dicot plant, strips of dry cobalt chloride paper, glass slides, clips. (OR) To prove unequal transpiration from the two surfaces of a dorsiventral leaf. To avoid excessive loss of water through transpiration dicot leaves possess more stomata on the lower surface as this surface is not much exposed to sunlight.Īim of the experimentTo prove more transpiration occurs from the lower (ventral) surface of a dicot leaf than upper (dorsal) surface. Stomatal pores get widely opened when the leaf get exposed to bright sunlight. So more transpiration occurs through the lower surface of the leaves of this plant. In dicot plants leaves will have more stomata on the lower surface than the upper surface. In all green plants transpiration occurs mostly through the stomata of the leaves. IntroductionTranspiration is the evaporative loss of water through the aerial parts of the plants especially through the leaves.
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